Effect of the dopamine antagonist, domperidone alone and in combination with thyroxine, clomiphene, hcg or gnRH on serum sex steroid profile, gonadal maturation and spawning of nile catfish, clarias lazera

The present investigation was carried out on one hundred and twenty mature Clarias lazera fish during the prespawning season [March - April]. Fish were allocated into two main groups [sixty fish of each sex] Fish weight ranged between 200-220 g. b. wt. Each main group was subdivided into six equal groups [Ten fish each], Fish of each group; both males and females were subjected to the same regimen of intraperitoneal exogenous treatment, as follows. 1[st] Group: Saline 0.65% NaCL solution. [Control] 2[nd] Group: Domperidone 5 microg/g. b. wt. in saline. 3[rd] Group: Domperidone 5 microg/g. b. wt, plus thyroxine 1 nancg / g. b. wt. in saline. 4[th] Group; Domperidone 5 microg/g, b. wt. plus clomiphene citrate l gamma g/g. b wt. in saline. 5[th] Group: Domperidone 5 micro g/g. b. wt. plus hCG 2 lU/g, b. wt. in saline. 6[th] Group: Domperidone 5 microg/g. b. wt. plus GnRH 10 microg/kg. b. wt. in saline. The present study revealed that [Domperidone] administration alone, in each sex of Clarias fish; increased serum sex steroid hormones [testosterone and 17 beta estradiol], gonadal weights [testes and ovaries], final gonadal maturation and gonadosomatic indices of both sexes as compared to respective controls. Exogenous administration of domperidone plus gonadotropin releasing hormone [GnRH] was the most effective treatment for production of sex hormones, final gonadal maturation and spawning in both sexes of Clarias lazera fish, followed by domperidone plus thyroxine; domperidone plus clomiphene citrate and domperidone plus hCG, respectively. In conclusion, the exogenous administration of dopamine antagonist plus GnRH is the most efficient regimen for enhancement of gonads maturation [gonadal recrudescence], spawning and spermiation of Clarias lazera fish throughout the prespawning season

Some analytical studies in relation to cyclicity of buffalo-Heifers

In this study, 40 non-cycling buffalo-heifers were used. The heifers were treated by exogenous sex steroids [Synchro-Mate-B] plus equine chorionic gonadotrophins [eCG] with or without carboxylic ionophore [Lasalocid] supplement for induction of estrus. Blood samples were collected from all heifers before treatment and from 10 positively responded heifers after treatment. Blood samples of 10 responded heifers [before and after treatment] in each group were subjected to study the effect of hormonal induction of cyclicity on metabolic profile testing. The results revealed that, the hormonal treatment without Lasalocid supplement did not alter Hb concentration, PCV%, ALT activity, total protein, albumin and urea nitrogen concentrations. While, the activities of plasma AST and ALP were significantly decreased and CPK activity, glucose and cholesterol concentrations were increased. Synchro-Mate-B implants plus eCG administration in Lasalocid supplemented cycling heifers did not lead to significant alterations in most of metabolic profile testing except PCV% and glucose concentration as compared to non-cycling buffalo-heifers supplemented with Lasalocid. Concentrations of plasma macro- and micro elements [Ca, Mg, Na, K and lithium] after induction of estrus by sex steroids plis eCG were not significantly differed in heifers either supplemented or not with Lasalocid. Thus, we can conclude that usage of Synchro-Mate-B implant plus eCG to induce estrus in prepubertal buffalo-heifers did not alter most of the metabolic profile parameters

Some metabolic hydrolases and blood chemistry values in plasma of Nile catfish Clarias lazera

In the present study some metabolic hydrolases and plasma chemistry values were studied in clarias lazera, the obtained results showed that: 1] plasma lipase level in male clarias lazera fish [2396.51 +/- 50.62 U/L] was significantly higher than that in female fish [1835.70 +/- 35.44 U1] at P<0.01. 2] Plasma amylase level in male fish [110.30 +/- 8.50 U/d] was significantly higher than that in female fish [76.70 +/- 4.82 U/dl] at P<0.01. 3] Plasma total protein level in male fish [5.50 +/- 0.35 gm%] was significantly higher than that in female fish [3.12 +/- 0.42gm%] at P<0.01. 4] Plasma glucose level in male fish [109.76 +/- 4.8 mg%] was significantly higher than that in female fish [88.43 +/- 3.93 mg%] at P<0.01. 5] Plasma total calcium level in male fish [7.85 +/- 0.31 mg%] was significantly elevated than that in female fish [5.87 +/- 0.50 mg%] at P<0.01. 6] Plasma inorganic phosphorus level in male fish [5.82 +/- 0.73 mg%] was significantly higher than that in female fish [3.77 +/- 0.52 mg%] at P<0.05. 7] No significant difference in plasma potassium level in both sexes of Clarias lazera fish

Dynamics of ovarian steroids in female Nile catfish [clarias lazera] during the different seasons and ages

The present investigation was done on 84 female Nile catfish [Clarias lazera] during the different seasons all over the year. The age of the fish was determined after preparation and examination of the third detachable vertebrae. Serum estradiol and progesterone concentrations were determined by using radioimmunoassay technique. The results revealed that maximal estradiol concentration was reported during spring [prespawning phase], while minimal concentration was recorded during winter [preparatory phase]. Serum progesterone concentration showed no significant variations during the different seasons and reproductive phases in all ages of female catfish. The influence of age on serum estradiol concentration is a season-dependent. It was concluded that variations in the concentration of estradiol 17-B in the blood may play an important role in ovarian activity in Nile catfish